Mr. Robert Wadley

B.App.Sci. Medical Laboratory Science, UTas: Grad.Dip.Sci. Microscopy & Microanalysis, USyd

Flow Cytometry Core Manager

Projects

About me

Education:Bachelor of Applied Science in Medical Laboratory Science,University of Tasmania, 1996Graduate Diploma of Science (Physics) in Microscopy & Microanalysis (Biological Applications),University of Sydney, 1999

Role:To operate and manage the flow cytometry instruments in order to maximise the efficiency and effectiveness of the equipment.To support staff including students in all aspects of flow cytometry.Develop new laser based applications in cytometry and advise Team Leaders and Scientific Manager on appropriate changes or developmental strategies.

Background:I started my science career as curator of the Rodda Pathology Museum, University of Tasmania at the end of 1992, I then spent several years in histology and electron microscopy laboratories at the Royal Hobart Hospital, Royal Prince Alfred Hospital - Sydney, CSIRO (Molecular Science) - North Ryde, and the University of NSW (Optometry). 

I learnt flow cytometry on a MoFlo MLS when I started as the Manager of the Cellular Analysis Facility (CAF) in the School of Microbiology & Immunology at UNSW in 1998.  The CAF was a semi-commercial unit and my clients included the Garvan Institute and Johnson & Johnson Research.  This facility contained:  Australia’s first MoFlo MLS cell sorter/analyser, Australia’s first Laser Scanning Cytometer (LSC) and a Bio-Rad MRC 1024 Confocal System. 

I was an invited speaker to the 2000 AFCG meeting in Adelaide speaking about the LSC. 

In 2001 I presented at the MoFlo User Group (MUG) meeting in Fort Collins, Colorado, detailing the wide variety samples that were analysed and sorted on the MoFlo MLS at UNSW. 

In 2001 I was ‘head-hunted’ by the Veterinary Faculty of the University of Sydney, & I ran their MoFlo SX for 14 months, the MoFlo SX is a dedicated sperm sorter used to isolate X carrying sperm for artificial insemination.  The MoFlo SX is the only instrument of its type in Australia, and was owned by XY Inc, Colorado.  I was an invited speaker to the 2001 AFCG meeting in Melbourne speaking about the special techniques involved in running a MoFlo SX. 

Mater Research Milestones:

Just prior to my commencement, MMRI had purchased a BD LSR II and FACSAria I.  

The next major instrument purchase was a Zeiss 510 Meta Confocal System (2006) and an UPS system (2006), followed by the High Throughput Sampler for the LSR II (2007).  

I was the chairman of the Australasian Flow Cytometry Group (AFCG) Education Committee from 2008-2011.  

Demand for a second multi-laser multi-colour analyser saw the purchase of a refurbished CyAn ADP (2009).   

Sabbatical supervision: Mr Zhang Wuwen, Chief Technician, Laboratory for Women’s Reproductive Health, Women’s Hospital Zhejiang University, Hang Zhou City, Zhejiang Province, China. (2009).  

The FACSAria I was upgraded to a FACSAria IIu (2009), a refurbished Beckman Coulter FC 500 replaced the FACSCalibur (2010). 

The FC 500 was modified to use a green laser (532nm) for mCherry experiments.   

During 2011 I acquired the first 2 laser scanning cytometers delivered to Australia, and from these I assembled a working instrument.  

Since 2011 I have been a member of the International Society for Advanced Cytometry’s (ISAC) Biosafety Committee.  

Sabbatical supervision: Dr Leonore de Boer - Manager, Cell Imaging Facility, Institute for Health and Biomedical Innovation, Queensland University of Technology (2012).

Publications

Publications at MR:

International Society for the Advancement of Cytometry Cell Sorter Biosafety StandardsKevin L. Holmes,1* Benjamin Fontes,2 Philip Hogarth,3 Richard Konz,4 Simon Monard,5 Charles H. Pletcher Jr.,6 Robert B. Wadley,7 Ingrid Schmid,8 Stephen P. Perfetto9Cytometry Part A: 00A: 00_00, 2014

Human CD1c (BDCA-1)(+) myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli.Kassianos AJ, Hardy MY, Ju X, Vijayan D,, Ding Y, Vulink AJ, McDonald KJ, Jongbloed SL, Wadley RB,, Wells C, Hart DN, Radford KJ.Eur. J. Immunol. 42, 1512 (2012), June 2012

Positioning of bone marrow hematopoietic and stromal cells relative to blood flow in vivo: Serially reconstituting murine hematopoetic stem cells reside in niches most poorly refused by circulating blood Jean-Pierre Levesque, Valerie Barbier, Robert Wadley, Andrew Zannettino, Sharon Williams, Ingrid Winkler. Blood. 22 July 2010; Vol. 116, No. 3, pp375-85

Human CD141+ (BDCA-3)+ Dendritic Cells Represent a Unique Myeloid Dendritic Cell Subset that Cross-presents Necrotic Cell Antigens.                                                                                                                                    

Sarah L Jongbloed, Andrew J Kassianos, Kylie J McDonald, Georgina J Clark, Xinsheng Ju, Catherine E. Angel, Chun-Jen J. Chen, P. Rod Dunbar, Robert B. Wadley, Varinder Jeet, Annelie JE Vulink, Derek NJ Hart, & Kristen J Radford. The Journal of Experimental Medicine, 2010 Jun 7;207(6):1247-60.

Published Abstracts:

Integrating different fluorescent technologies in a flow cytometry laboratoryRB Wadley (2005 ISAC Samuel A. Latt Conference, oral abstract)Cytometry Part A, Volume 69A, Issue 5, May 2006

Book Chapters:

T cell Transfer model of colitis: A great tool to assess the contribution of T cells in chronic intestinal inflammationAuthors:  Rajaraman Eri, Michael A. McGuckin & Robert WadleyMethods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2012, Volume 844, p. 261, January 2012

Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow in VivoAuthors:  Valérie Barbier, Ingrid G. Winkler, Robert Wadley, Jean-Pierre LévesqueMethods in molecular biology (Clifton, N.J.), ISSN 1064-3745, 2012, Volume 844, p. 45, January 2012

Conference Presentations:

28-30/11/2013, AFCG, Wellington, New Zealand (Poster)The New ISAC Cell Sorter Biosafety Standards: How did they contribute to my new facility design and instrument choices?Robert Wadley & Dalia Khalil

24-26/8/2011, AFCG, Hobart, TasmaniaRebuilding one of Australia’s first two Laser Scanning Cytometers: How to benefit from old technologyRobert Wadley

24-26/8/2011, AFCG, Hobart, Tasmania (Poster)Setting up a Core Facility with 100% Cost Recovery: A Personal PerspectiveRobert Wadley

24-26/8/2011, AFCG, Hobart, Tasmania (Poster)Cell Counters: Useful instruments in a flow cytometry laboratoryRobert Wadley

8-10/11/2010, AFCG, Sydney, NSW (Poster)An inexpensive way to allow Fluorescent Protein Evaluation in a Cytometry FacilityRobert Wadley, Dalia Khalil

07/08/2009, Mater Medical Research Institute’s Dendritic Cell Symposium, Des O’Callaghan TheatreOptimising a digital multi-colour flow cytometer for a humanised NOD/SCID mouse model

R Wadley

14/08/2008, AFCG, Fremantle, WA8 colour sorting with manual compensation of haematopoietic stem cells on a FACSAria.                                                                                                                                                                                                                               

Jean-Pierre Levesque, Ingrid Winkler, Robert Wadley

13-15/08/2008, AFCG, Fremantle, WA (poster)Looking to the future – Cytometry Traineeships?                                                                                                                                                                                                                                                                                                       

Wadley R, Clark G

17-21/05/2008, ISAC, Budapest, Hungary (poster)Looking to the future – Cytometry Traineeships?                                                                                                                                                                                                                                                                                                       

Wadley R, Clark G

18/09/2007, AFCG Melbourne, VictoriaDual Emission of Hoechst 33342 from a Violet Laser to Define Stem CellsJ-P Levesque, I Winkler, R Wadley

08/11/2005, ISAC/AFCG/ASCC, Gold Coast, QldIntegrating Different Fluorescent Technologies in a Flow Cytometry LaboratoryR Wadley