TRI PROTEOMICS FACILITY

The Proteomics Facility operates as a core facility within the Translational Research Institute (TRI) with The University of Queensland Diamantina Institute acting as the service provider.  The facility operates at cost recovery to provide specialised service for TRI researchers and external users.  The facility also provides training and access to specialised equipment for sample preparation, sample analysis and data analysis.

WHAT IS PROTEOMICS?

The study of proteins in a system

Modern proteomics techniques use Mass spectrometry for protein identification and  quantitation.  Proteomics can be used for protein characterization through sequence and post-translational modification analysis

Protein identification

Proteomics experiments can identify 1 to 1000s of protein in a single experiment. Each protein will have species, protein name and accession number information.  Protein identification is dependent on the protein already being present in a database.  Further, low protein abundance need optimization. 

Protein quantitation

Proteomics can provide quantitative information through mass spectrum numbers and intensities, number of unique peptides and protein coverage % information. Proteomic search engines will also provide protein ID confidence and false discovery rate info.  

How Can Researchers Use Proteomics?

The following diagrams explain the various ways researchers can use Proteomics services at TRI. 


Identifying proteins and expression changes between different treatment conditions


Identifying protein interactions


Characterizing protein modifications

Proteomics Facility Services

Researchers can use the services of the Proteomics facility in 2 ways - we have facility assisted services or self serve instrument access. 


A. Facility Assistance: Facility provides specialize services and training for proteomics sample analysis which include sample preparation, mass spec analysis and proteomics data processing. 


B. Instrument Access: Researchers are welcome to use facility instruments for sample preparation, mass spec analysis and proteomics data processing

User requirements

Researchers can organize instrument induction, training and booking through proteomics facility ppms. (https://ppms.int.tri.edu.au/login/?pf=11)

Researchers have to provide own HPLC columns for instruments 5, 6 and 7 shown below

 

2017 Proteomics Toolbox Talks 

To book your proteomics training session, head to the Proteomics Facility PPMS
> https://ppms.int.tri.edu.au/login/?pf=11, Click Order, Training, Toolbox
 
 

Toolbox Talks

Please note, the next toolbox talk program is being planned for October. Please stay tuned for more details. 
 
 
 

2017 Proteomics Internships

Open to TRI based students, the proteomics internships are offered twice per year. Please stay tuned for details about the next offering. 
 
 
 

New Instrument

Q-Exactive Plus

To discuss how the facility can benefit your research or for technical advice please contact the TRI Proteomics Core Facility Manager.

Dorothy Loo, MSc, B BioMed 

Level 5E, The University of Queensland Diamantina Institute
Translational Research Institute, 37 Kent Street, Woolloongabba QLD 4102

T: +61 7 3443 7066, E: [email protected]

PPMS: https://ppms.int.tri.edu.au/login/?pf=11

PROTEOMICS FACILITY INSTRUMENTS

The facility has the following instrumentation and capabilities:

Sample Preparation

1

Bioruptor Pico: Tissue homogenization and DNA fragmentation

2

Direct Detect: IR based protein and peptide quantitation

3

Off-gel Electrophoresis: Isoelectric focusing of analytes. 2D gels

4

Bravo robot: liquid handling robot for sample preparation

Sample Analysis

5

Stand alone HPLC: Analyte separation and detection

6

LC-QQQ: mass spec based quantitative protein analysis

7

LC-QTOF: mass spec qualitative protein analysis

8

Easy LC – Q Exactive Plus: New high-resolution, accurate-mass (HR/AM) orbitrap based mass spectrometer for quantitative and qualitative protein analysis

With the new Q-Exactive Plus mass spectrometer, researchers benefit from:

•50% reduction in sample preparation time
•90% reduction in sample input
•60% reduction in analysis time
•35% reduction in facility assisted sample analysis cost
•Facility also provides HPLC column with Q-Exactive Plus instrument use.  This means researchers can save $1500 on initial set-up costs.  

> Got questions? CONTACT DOROTHY LOO, PROTEOMICS FACILITY MANAGER ON (07) 3443 7066 OR EMAIL [email protected]

> Visit PPMS to book or create a booking account: https://ppms.int.tri.edu.au/login/?pf=11