Projects
Diagnosis and treatment of liver diseases by real-time imaging and nanotechnology
Liver diseases, particularly viral hepatitis, cirrhosis and hepatocellular carcinoma, are common in clinical practice with high morbidity and mortality worldwide. This study aims to assess the application of multiphoton microscopy with fluorescence lifetime imaging (MPM-FLIM) in common liver diseases for diagnosis and observing therapeutic effect. Normal and diseased mice livers (liver with steatosis, fibrosis, hepatocellular carcinoma, and ischemia-reperfusion injury) will be imaged by MPM-FLIM. The acquired MPM-FLIM images will be compared with conventional histological images. Cancer cells will be marked with quantum dots, and then transplanted to nude mice to mimic circulating tumour cells (CTCs). The spatiotemporal disposition of doxorubicin in liver will be observed by MPM-FLIM. The preliminary results indicated that MPM-FLIM could simultaneously image and quantify the cellular morphology and microenvironment of diseased livers in mice models without conventional biopsy or fluorescent dyes. It can also reveal the spatiotemporal disposition of free doxorubicin in liver with hepatocellular carcinoma. This study will demonstrate for the first time that MPM-FLIM is able to image and quantify the cellular morphology, microenvironment and cells/drugs spatiotemporal disposition in liver.
About me
Haolu Wang received his BS degree in clinical medicine and master degree in surgery with a MD certificate from School of Medicine, Shanghai Jiao Tong University in 2009 and 2011. He had been a resident surgeon of Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University from 2011 to 2013. Since 2014, he has been pursuing a PhD degree in School of Medicine, the University of Queensland. His current research interests include animal models of liver disease, nanomaterials for diagnosis and drug delivery, and audit systems in surgical treatment of hepato-biliary-pancreatic cancer.
Publications
Wang, Haolu, Liang, Xiaowen, Mohammed, Yousuf, et al. Real-time histology in liver disease using multiphoton microscopy with fluorescence lifetime imaging. Biomed Opt Express. 2015, 6, 3.
Wang, Haolu, Thorling, Camilla, Liang, Xiaowen, et al. Diagnostic imaging and therapeutic application of nanoparticles targeting to the liver. J Mater Chem B. 2015, 3, 939.
Wang, Haolu, Chen, Tao, Wang, Hui, et al: A systematic review of the Physiological and Operative Severity Score for the enUmeration of Mortality and morbidity and its Portsmouth modification as predictors of post-operative morbidity and mortality in patients undergoing pancreatic surgery. Am J Surg. 2013 Apr;205(4):466-72.
Wang, Haolu, Li, Xinxing, Chen, Tao, et al: The mechanisms of verapamil-enhanced chemosensitivity of gallbladder cancer cells to platinum drugs: glutathion reduction and MRP1 downregulation. Oncol Rep. 2013 Feb;29(2):676-84.
Wang, Haolu, Li, Kewei, Wang, Jian: Sclerosing angiomatoid nodular transformation (SANT) of the spleen: report of five cases and review of literature. Chinese Med J. 2012; 125(13):2386-2389.
Wang, Haolu, Li, Kewei, Wang, Jian. A large lipoma of the pancreas. ANZ J Surg. 2011; 81(3): 200.
Yang, Linhua, Wang, Haolu (co-first author), Liang, Xiaowen, et al: Bacteria in hernia sac: an important risk fact for surgical site infection after incarcerated hernia repair. Hernia. 2014. Jun 13. [Epub ahead of print].
Wang, Hui, Wang, Haolu (co-first author), Liang, Xiaowen, et al: Evaluation of the POSSUM, P-POSSUM and E-PASS scores in surgical treatment of hilar cholangiocarcinoma. World J Surg Oncol. 2014. Jun 24; 12:191.
Chen, Tao, Wang, Haolu (co-first author), Wang, Hui, et al: POSSUM and P-POSSUM as predictors of postoperative morbidity and mortality in patients undergoing hepato-biliary-pancreatic surgery: a meta-analysis. Ann Surg Oncol. 2013; 20 (8): 2501-10.
Jin, Yue, Wang, Haolu (co-first author), Liang, Xiaowen, et al: Pathological and prognostic significance of hypoxia-inducible factor 1α expression in epithelial ovarian cancer: a meta-analysis. Tumor Biol. 2014 Aug; 35(8):8149-59.
Thorling C, Jin L, Weiss M, Crawford D, Liu X, Burczynski F, Liu D, Wang H, Roberts M. Assessing Steatotic Liver Function after Ischemia-reperfusion Injury by In Vivo Multiphoton Imaging of Fluorescein Disposition. Drug Metab Dispos. 2014 Nov 7.
Li, Xinxing, Dong, Ying, Wang, Wei, Wang, Haolu, et al: Emodin as an effective agent in targeting cancer stem-like side population cells of gallbladder carcinoma. Stem Cells Dev. 2013 Feb 15;22(4):554-66.
Li, Xinxing, Wang, Jian, Wang, Haolu, et al: Characterization of cancer stem-like cells derived from a side population of a human gallbladder carcinoma cell line, SGC-996. Biochem Bioph Res Co. 2012. 23;419(4):728-34
